For the last week I’ve been working on this little project, where I’ve found a group of wide-field neurons, that have synapses only or mostly in the medulla. My goal was to completely cover each of the medulla’s layers.
The cells, I’ve chosen, are here (thanks @st0ck53y for all the Dm4s):
The next step was to use the Connectivity app and collect all the upstream and downstream partners of these cells. IIRC, there was over 270k segments. Using Notepad++, I’ve removed all the duplicates and thinned the list down to around 170k segments. Then I’ve sorted the remaining IDs in the ascdending order.
The next step was the most tedious one. I was copying the IDs in batches of around 1000 to FW and selected manually all the cells that looked completed (FW doesn’t show the status, when there are more than 60 cells at the same time), almost completed or at least are some significant parts or cells. I’d say, over 100k of the cells were tiny fragments/twigs. During this selection I’ve also got rid of most of the big glia mergers. The result is a list of over 27k cells.
All the IDs are available here:
The reason, I’ve done it, is to find all cells of all types and identify them. If the method will be good, I might do something similar for the Lobula and Lobula Plate in the future.
Currently I’m looking for all the Lawf cells. After that, I’m planning to split the results further to 3 groups: cells, that project outside the optic lobe; cells, that projects outside the medulla; and cells, that are completely within the medulla.
If I didn’t miss any big volume, the cells, that should be in the list are: L, C, Lawf, Mi, Mt, Tm, TmY, T1 - T4, R7 & 8, Dm, Pm, Y, Olt and probably some others.
If anyone wants to join me in this searching, wants to prepare similar list for other neuropils or the other optic lobe, feel free to join
If you want to go through the list, I’d suggest to work on about 500 cells at a time (unless your computer can handle more, of course).