Sorry I have been a bit MIA - I broke my arm last week ugh!!
I’d like to ask, what informations can scientists infer from such a narrow (flat?) dataset? I don’t negate the usefulness of it, not at all. I’m just curious, what can be found by analyzing this data.
P.S. Get well soon, @Amy_R_Sterling !
It’s true that the dataset is slim. Still, it is novel to reconstruct synaptic inputs to the CA3 PyCs. In many cases the axons are distinctive enough to know what cells or brain region the came from. Of particular interest are mossy fibers. Bouton size distribution (connectivity strength) is being analyzed between mossy fibers and PyCs. Vesicles are being segmented as well. We can also analyze in detail the connectivity of the interneurons in the dataset. Zhihao can possibly share more. The preprint is still in progress.
what/how do we label a row/segment in the gsheet if we have a pyramidical cell in there (along with other cells)? Done or something else?
It’s fine to label these as “Done.” I’m going to start a new thread for general help requests as well
we may or may not be out of cell entries in the gsheet
there are still the two demo cells
doesnt M need them for future demo-ying?
don`t know, would think she could use any random cells
We’re in process of getting more
Also for the future these kinds of questions are well suited to the Pyr Proofreading Help thread. Let’s leave this one for science question!
Haven’t seen it in BANC (or FW) or at least far away from the dataset end, but how come/why are there such regions in pyr’s current dataset where stuff are untraceable? They appear to be ‘cubes’ of it as well shape wise.
I’d attach a vid but vid formats aren’t allowed.
I’ve seen this in several places around the dataset.
this one’s around 44000, 36276, 2107.
thanks.
Is there a defined end goal for this dataset or are we just identifying as many MF candidates as possible?
Oh I guess additionally how does the size (of the files composing this dataset or the physical size) compare to the other ones we’ve worked on
i have not seen places where it cut of like that, other than when there seems to be some kind of loading trouble on my end. But it looks like there are more of unselectable stuff than in banc/flywire from my experience so far.
Decided to put this one in questions versus amusements. This is the first time I’ve seen a neuron here take a directional reversal, and amusingly, draping over and around another neuron to do so:
https://play.pyr.ai/#!middleauth+https://global.daf-apis.com/nglstate/api/v1/5755220396081152
Its direct siblings do not seem to take this kind of directional approach. Naturally, I’m curious!
I’ve seen something similar in Flywire. I believe, it has to do with the development phase, when the neurons are in quite different positions and move in all directions. Sometimes they twist like this and then the “stronger” one wins this rope pulling contest when they move to they target positions.
Is fly’s equivalent of hippocampus the mushroom body or something else, or there isn’t any analogue?
https://play.pyr.ai/#!middleauth+https://global.daf-apis.com/nglstate/api/v1/6328861865279488
what even is this cell? I’ve seen less twists, turns and loop de loops in Agatha Christie novels!
No, srly what type of neuron is this cell? It reminds me of the ‘bouquet’ cells in FW fly brain that would circle around break off into many sub branches and end up in the ‘bouquet of flowers’ kind of thing, but this is no fly brain.
This may have been answered in one of the town halls/zoom calls but how come is the dataset we’re currently working in like a smaller dataset inside a bigger dataset? Is the larger one going to be available later on or is it blocked off for some other reason?
Anyone can feel free to finish those two “demos” from Challenge 1 if you’d like!