Gallery of amusements: FlyWire edition!

Decide to do a few Dm4’s as a break from the hallowxmas grind, and return to see this monstrosity that i never finished before:

https://ngl.flywire.ai/?json_url=https://globalv1.flywire-daf.com/nglstate/5081663281299456

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Slowly but surely, slice by slice, the outer lobula plate neurons are being sorted. This EM slice is right at the edge of the data set outside the lobula plate, and it just gets more dense the closer in it gets. I know some of you have a lot more time to work on Flywire than I do, but I’m still proud of how far I’ve come in a couple of days.

For something more colorful, have a cluster of the T4 cells I’ve found so far in this grouping:

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nice farm! T2s and 5s were/are my favourites, my farm started with me wanting to find them all, lol, now its at 5.2k cells lol.

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If you have a list of 5,000 cells (or even a couple hundred) that are all the same type/known type we can batch label them!

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https://ngl.flywire.ai/?json_url=https://globalv1.flywire-daf.com/nglstate/5885232167256064

Was looking at downstream connectivity of DM8s and it went really crazy downstream from this cell - visual projection neuron labeled aSP29a. That point that the signal goes BOOM!
asp29a has such nice symmetry!

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I’ve finished going through identifying and proofreading the outermost slice of the lobula plate (the lobula plate cell body rind), and thought I’d share findings and images!

As you can see here, most of the somas in this outermost range are T5 types. There are approximately 214 identified T5 cells on this layer (approximate because a few I have identified are a little further in). Link: FlyWire


Only about 27 cells are T4 type. Link: FlyWire


About 10 are Y type. Link: FlyWire


Three are Tlp type: FlyWire


6 are Lpi type: FlyWire


And 5 are LLPC type: FlyWire


Finally, I have 8 neurons that I will post in “What Cell Is It?” that I could not classify or reasonably complete. Assistance on these would be appreciated!

That makes a total of about 271 cell types on this outer EM slice. What’s next? Well, I shuffle on up a few EM slices and start identifying the next layer! There will be a lot more somas as I head further in, but it’s interesting to see how the lobula plate exterior is forming.

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This is incredible, AzureJay! It must be so satisfying to have completed that whole layer :heart_eyes:

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It is so much! Even if it’s only the beginning, it’s so good to see a full slide processed.

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Wow this is so cool!

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All the C cells, I’ve completed:

https://ngl.flywire.ai/?json_url=https://globalv1.flywire-daf.com/nglstate/4777855682609152

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I wonder, what are those additional branches in these T cells (bottom left quarter of the pic). I’ve checked over 1000 of them and only these two have such extensions going around the Lobula.

https://ngl.flywire.ai/?json_url=https://globalv1.flywire-daf.com/nglstate/4808773340233728

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Wowww this is amazing!!

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Emil Kind has to this to say of these wild neurons: “I suspect some developmental variability. I also rarely see similar branching on other neurons. I could imagine that these are remnants of filopodia that are normally eliminated during development, but survived in this case.”

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outdated cell id

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Almost all my T4 cells colored by layer:


https://ngl.flywire.ai/?json_url=https://globalv1.flywire-daf.com/nglstate/4664830094999552
Looks, that the Giant Neurons method works quite well :slight_smile:

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Interesting T cell with additional terminals along the axonal neurite:

https://ngl.flywire.ai/?json_url=https://globalv1.flywire-daf.com/nglstate/6443025760780288

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Almost all T4 and T5 neurons in the left LOP:

T4:

https://ngl.flywire.ai/?json_url=https://globalv1.flywire-daf.com/nglstate/6422230066003968

T5:

https://ngl.flywire.ai/?json_url=https://globalv1.flywire-daf.com/nglstate/4696109788168192





Edit:
Because there’s a limit to at most 3 consecutive posts, I’m editing the previous one to add a new finding:

According to FlyBase there are approximately 3 aMe12 cells in each optic lobe. Looks, that our fly has at least 4 of them in the left LOP:

https://ngl.flywire.ai/?json_url=https://globalv1.flywire-daf.com/nglstate/5432669601005568

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Ill break the 3 post streak with the whole left lobe Dm4 layer (minus two boutons, still looking for them). Right lobe in progress, should be done before new year :partying_face:


https://ngl.flywire.ai/?json_url=https://globalv1.flywire-daf.com/nglstate/5933814438166528

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Wow, that looks incredible!

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All the left Lawf1 I was able to find so far:

https://ngl.flywire.ai/?json_url=https://globalv1.flywire-daf.com/nglstate/4865346112585728

There are still some holes, but I’d say, it’s over 95% of all the cells in this lobe (a few of them are still incompleted).

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