Thanks @Amy_R_Sterling! 
In the mean time, I’ll be working on the other cells from similar groups (still have to identify them).
And yes, both these and Lawf cells create really nice nets, showing where all the cartridges are. It also helps with finding the missing ones, where the net doesn’t seem to be complete.
I’ve found more of these supposedly Mi10 cells:
https://ngl.flywire.ai/?json_url=https://globalv1.flywire-daf.com/nglstate/6412837945606144
They look like the Mi10 from the Fischbach.
However, this one looks a little different (the bottom part):
And now I don’t know, what to think.
hm from that picture it looks like the first arbour is in layer 1 or 2 so would think it is closer to a mi12 from fishback?
Yeah, that’s what I’m thinking too - their Mi10 is actually Mi12 from Fischbach.
Now, I have to find an actual Mi12, to have all three types available for comparison.
Thanks
I was looking for Mi12, but instead found:
Mi14:
https://ngl.flywire.ai/?json_url=https://globalv1.flywire-daf.com/nglstate/6717743384494080
Mi2 (?):
https://ngl.flywire.ai/?json_url=https://globalv1.flywire-daf.com/nglstate/5210004705509376
And some other Mi type, which I wasn’t able to identify:
https://ngl.flywire.ai/?json_url=https://globalv1.flywire-daf.com/nglstate/5183030303916032
I agree about your classification, but too me it looks like your third link also is Mi14 why have you made those out too be a different cell?
Mi14 should have two main branches in the bottom part, while these have only one branch there.
The ones you have classified as Mi14 have 2,3 or 4 main branches too the bottom if having one branch too the bottom mean it is a different cell i would guess it is Mi 14 a,b,c,d.
Hmm, indeed, some of them do have more than 2 branches. I’ve seen it, but didn’t thing anything about that.
You might be right with subtypes. Because Mi14 and these from the third link are the only Mi’s, that have 3 distinct layers of synapses.
Mi7 ?
https://ngl.flywire.ai/?json_url=https://globalv1.flywire-daf.com/nglstate/5890183845117952
Dm2 ?
https://ngl.flywire.ai/?json_url=https://globalv1.flywire-daf.com/nglstate/5389618358779904
Dm7 ?
https://ngl.flywire.ai/?json_url=https://globalv1.flywire-daf.com/nglstate/5735667363479552
My best guess is Dm 2 on both of the first two links and Dm 7 on the last
I’m quite surprised, that you think, that both first links are of the same type. Maybe that one cell floating alone misled you, because it actually was a Dm2. The other ones in that first first are actually quite different from that one (or maybe I’m seeing things, that are not there, lol).
Also, I have another problem. I’ve found 2 types of multi-layered cells, I’m not sure, which is which.
I suppose, one of them is a set of Mi3s and the other one - Dm7s, but don’t know, which is which. Or maybe something else, entirely.
https://ngl.flywire.ai/?json_url=https://globalv1.flywire-daf.com/nglstate/6132070497648640
https://ngl.flywire.ai/?json_url=https://globalv1.flywire-daf.com/nglstate/5225493968191488
And now I think, the Dm2s can also be Dm5s or Dm8s.
I think, I’m starting to loose my mind with these cells, lol.
yes, you are ofc correct, i suppose i did look at them and possible changed up the tabs thinking i was looking at both when really looking at only the dm2 link.
I suppose the ones you though could be Mi7 could be Dm5? Dm16 is also a possibility but is supposed to be higher in the medula.
i am pretty sure this is Dm8 FlyWire
As for the multilayered cells if one of them is Mi3 and the other Dm7 the first is Mi3 (have small twigs on the main steam) but not at all sure it is only the two of them it can be, might be others we have little information on.
Ok, thanks for clearing that up.
Agree about the Dm8.
I will look for the lesser known types of Mi and Dm, because the ones with possible 3 layers, look kinda different, than anything from the Fischbach.
I have a problem with LC10 cells.
There are 2 subtypes: 10A and 10B. They can be split further:
10A → 10b, 10c
10B → 10a, 10d
However, I can’t tell any differences between the cells.
Here’s a link with several LC10 neurons (many still missing):
https://ngl.flywire.ai/?json_url=https://globalv1.flywire-daf.com/nglstate/5158623950929920
The descriptions of the subtypes are as following:
10a
Subtype of lobula columnar neuron LC10B. It has its main arbors in lobula layers 3, 4 and 5B, with some processes extending into layer 2 and some branches in layers 5 and 6. Presynaptic sites are observed in layer 3. It is a cholinergic neuron (Davis et al., 2020).
10b
Subtype of lobula columnar neuron LC10A. It has its main arbors in lobula layers 4 to 6, with arbors denser in the latter, and many parallel processes exist between the two layers. Presynaptic sites are mainly observed in layer 6, with some in layers 4 and 5A. It is a cholinergic neuron (Davis et al., 2020).
10c
Subtype of lobula columnar neuron LC10A. It has its main arbors in lobula layer 5B, some branches in 6 and some processes reaching into 5A. It projects to the most lateral of the dorsal optic glomeruli of the PVLP, ventral to LC16. There are around 70 cells of this type.
10d
Subtype of lobula columnar neuron LC10B. It has its main arbors in lobula layers 4 to 6, with less dense arbors in 5B than in layers 4, 6 or 5A (in contrast to LC10a). Presynaptic sites are observed in layers 4 and 6 (less numerous than LC10a in layer 3 and LC10b in layer 6). Its arbor is much smaller than LC10b. There are around 84 cells of this type.
Source: FlyBase
If anyone could find a few examples of each subtypes, that would be great.
Edit:
Here’s an image showing the differences:
10a an 10d FlyWire purple cell have synapses in layer 3 and 5 and should be a 10a. The red cell have synapses in 4 and 6 and based on elimination have to be 10d
10b FlyWire most incoming synapses in layer 6
10c FlyWire only in layer 5 and 6
I have tried too group a few of the cells and is pretty sure about red beeing 10a and blue beeing 10c
i think purple might be 10b and really unsure about green possible beeing 10d
https://ngl.flywire.ai/?json_url=https://globalv1.flywire-daf.com/nglstate/6114365709221888
Thank you! I’ll try to follow your examples.
I’m trying to identify some LC cells in the left optic lobe.
Here are my findings:
https://ngl.flywire.ai/?json_url=https://globalv1.flywire-daf.com/nglstate/6641683540213760
My guesses are:
LC24 - orange
LC25 - purple
LC26 - green
There are also yellow cells in a hidden tab. They also look similar, but I can’t identify them.
I’m using this paper as my base for the identification.
Especially these 2 images:
I wonder, what others think about this identification. Is this correct? Maybe some (all?) of these neurons belong to some other types?
I have trouble deciding, which TmY cells these are:
My candidates are TmY3 and TmY8. The top part looks more similar to TmY3, but my cells don’t have two protrusions, while the ones in Fischbach do have. TmY8 have one, but top looks a little bit different.
i would say most likely a TmY3
the part going into lobula is have the right length and is narrow like TmY3 while TmY8 looks like it is branching more and longer in lobula.
as for the branch going into lobula plate fishback suggest TmY3 go trough the plate to sector 4 while your cells looks they only go too layer 3
As for the two protrusions maybe this is a anomaly or that TmY3 can have different variants
