Quest for the Missing Photoreceptors

While most eye cartridges contain 6 R1-6 cells with an R7 and an R8, we’ve learned that there are a couple of regions/areas where there may be some differences.

The R cell number varies slightly in two locations:

  • Equator
  • Edge

This is due to neural superposition during eye development. At the equator, there are 4 rows of cartridges with 7 or 8 R1-6. At the edges they can have less, sometimes also without R7/8.

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I feel confident enough to declare sector 4 complete!

I changed my approach midway through this sector and moved to identifying photoreceptors by cluster (L1+R1-8) and found it a lot easier to follow in this sector. Also noticing a few distinct shape patterns in R1-6 that may help in further narrowing these types down, though I haven’t taken any deep dive into those patterns.

Sheet is updated and here’s a handy link here: FlyWire

(Going to take a small break out of these sectors to go back over my right lobe T4-T5 cells/cell farms but from here on out I am going to try to alternate work on that and photoreceptors since there’s many to go!)

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Huzzah! :tada: :partying_face: :partying_face: :partying_face: :partying_face: :confetti_ball:

Congrats on finishing Sector 4 @AzureJay! I’m glad you found the L1+R1-8 method a little easier. I also prefer to use the L1 cell as a major touchstone for finding certain cell types in a cluster/ommatidium.

PS. Let us know if you have any questions about your T4 or T5 cells – happy to help :slight_smile:

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Bravo, that’s wonderful!

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another 100 clusters of retinal cells from the right lobe done, this time in the area not affected by the rift, with my 200 clusters i have a band that goes almost from one end of the lobe to the other.





all id`s in each cluster can be found here cell farms - Google Sheets

and annotation points here FlyWire

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Beautiful R-cells @annkri! They even look like streamers of celebratory confetti :tada: :confetti_ball: :tada:

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As part of a joint effort from Flyers, Princeton lab members and researchers, the Photoreceptor Zone Quest has been completed!

Together, we proofread a total of 5k+ retinula cells in the right optic lobe using a variety of mapping processes (ie. spatial zones, common synaptic partners) to ensure full coverage. Thank you for your contribution and efforts to this Quest. We are now casting a wider net and focusing on tagging cells in the visual system. Happy Flying!

PS. If you’d like to attempt loading all 5k+ photoreceptors, here’s the link: neuroglancer (warning: this link takes really long time to see loading, be patient :slightly_smiling_face: )

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Thanks so much everyone. For science!

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after looking at the Dm8 cells classified by kk in the cell farms there is a lot of cells not marked as identified and i guess this might be the case in the other cell farms also. i am thinking the fastest way to get many cells is too look at the cells farm and identify the cells that did not get identified the first round

we should probably also have a system so not everyone is working on tagging the same type

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As for the Dm8, I wasn’t 100% sure about all the identifications, so I didn’t label them (hence the purple field in the “Status” column). If the field is green, that means, that I (or someone else) have already labeled (almost) all cells.

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ah okay, i am seeing it now, did have very little time too look at it this morning so forgot too look at the colour in the sheet.
i was just wondering on the difference betwheen the tagging dashboard and cell farms on a few but a big part of the explanation could be there then.

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Responded to your comments/questions about the Tag Rally here in this thread.

Cheers,
M.

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Another sector of 100 R clusters done, this one did go really fast since most of the cells are completed and a lot of them already identified. But still 290 cells was missing identification in this batch.

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